R- and G-band patterns in Astyanax scabripinnis paranae (Pisces, Characiformes, Characidae)
Genet. mol. biol
;
22(2): 201-4, jun. 1999. ilus
Article
in English
| LILACS
| ID: lil-242201
ABSTRACT
The absence of longitudinal bands in fish chromosomes has been associated with technical problems in chromosome preparations or the absence of a structural compartmentalization in the fish genome. In the present study, a R-banding pattern was obtained using a replication banding technique by in vivo treatment with 5-bromodeoxyuridine (5-BrdU). G-banding patterns were obtained after trypsin treatment and also after chromosome cleavage by in situ treatment with the restriction endonuclease BamHI. A similar G-banding pattern was also obtained after cleavage with the endonuclease HinfI. Presence of a resolute R- and G-banding patterns shows that Astyanax scabripinnis paranae chromosomes could present an isochore-like structure similar to that found in other vertebrates.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Chromosome Banding
/
Fishes
Limits:
Animals
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
1999
Type:
Article
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