Autoanticuerpos anti-tiroglobulina: detección y posible interferencia en la medición de tiroglobulina en suero / Thyroglobulin autoantibodies: detection and possible interference in serum thyroglobulin measurement
Rev. méd. Chile
;
127(6): 667-74, jun. 1999. graf
Article
in Spanish
| LILACS
| ID: lil-245308
ABSTRACT
Background:
Thyroglobulin measurement is useful for the follow up of patients subjected to total thyroidectomy for differentiated thyroid carcinoma. Thyroglobulin autoantibodies may interfere with its determination.Aim:
To measure thyroglobulin autoantibodies and their interference with thyroglobulin determination. Material andmethods:
The presence of thyroglobulin autoantibodies was investigated in 801 serum samples sent to the laboratory for measurement of thyroglobulin levels. A serum was considered positive for these autoantibodies when radioactivity corresponding to 125I-thyroglobulin bound to thyroglobulin autoantibodies, precipitated with human gamma globulin, exceeded in 1.4 times that of a negative sera pool. In positive sera, thyroglobulin autoantibody concentration was measured and its interference with thyroglobulin radioimmunoassay was assessed through a recuperation test using exogenous thyroglobulin.Results:
Thyroglobulin autoantibodies were detected in 149 sera (18.6 percent). Of these, 65 had a recuperation that fluctuated between 1 and 80 percent. Thyroglobulin autoantibody concentration was negatively correlated with recuperation percentages (r= -0.64; p <0.001) but not with thyroglobulin concentrations (r= 0.08). Thyroglobulin was higher in positive sera with a recuperation over 80 percent than in sera with a recuperation of less than 80 percent (12.7 ñ 1.7 and 5.9 ñ 0.6 ng/ml, respectively; p <0.001).Conclusions:
Thyroglobulin autoantibodies interfere with thyroglobulin measurement by radioimmunoassay, sequestering variable amounts of thyroglobulin. The presence of these autoantibodies must be investigated prior to thyroglobulin determination
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Index:
LILACS (Americas)
Main subject:
Autoantibodies
/
Thyroglobulin
Type of study:
Diagnostic study
Limits:
Humans
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
1999
Type:
Article
Affiliation country:
Chile
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