Cellulose acetate as solid phase in ELISA for plague
Mem. Inst. Oswaldo Cruz
;
95(1): 95-6, Jan.-Feb. 2000. graf
Article
in English
| LILACS
| ID: lil-251319
ABSTRACT
Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 µg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plague
/
Yersinia pestis
/
Cellulose
/
Antigens, Bacterial
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2000
Type:
Article
Affiliation country:
Brazil
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