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Use of remazol blue dyed avicel for the determination of cellullolytic activity in basidiomycetes
Correia, Marcos José; Pereira Junior, José Antônio de Souza; Santos, Jefferson Cunha dos; Cavalcanti, Maria Auxiliadora de Queiroz.
  • Correia, Marcos José; Universidade Federal Rural de Pernambuco. Departamento de Morfologia Animal.
  • Pereira Junior, José Antônio de Souza; Universidade Federal Rural de Pernambuco. Departamento de Morfologia e Fisiologia Animal.
  • Santos, Jefferson Cunha dos; Universidade Federal Rural de Pernambuco. Departamento de Morfologia e Fisiologia Animal.
  • Cavalcanti, Maria Auxiliadora de Queiroz; Universidade Federal de Pernambuco. Departamento de Morfologia e Fisiologia Animal.
Rev. microbiol ; 29(4): 286-8, out.-dez. 1998. graf
Article in English | LILACS | ID: lil-251738
ABSTRACT
A modified method for direct determination of cellulolytic activity using Avicel colored with Remazol Brilliant Blue R (RBBR) in Agar test tubes in discussed. Refinaments were introduced in a simple method for qunatitation of cellulase activity, based on the release of dye from Avicel-RBBR medium by enzimatic hydrolysis. Modification in Avicel-dye preparation were enhanced and a spectrophotometer improved the precision of the collected data, since absorbance measurements could be done at the maxmum wavelenght for RBBR (595 nm).
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Full text: Available Index: LILACS (Americas) Main subject: Basidiomycota / In Vitro Techniques / Coloring Agents Language: English Journal: Rev. microbiol Journal subject: Microbiology Year: 1998 Type: Article Affiliation country: Brazil

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Full text: Available Index: LILACS (Americas) Main subject: Basidiomycota / In Vitro Techniques / Coloring Agents Language: English Journal: Rev. microbiol Journal subject: Microbiology Year: 1998 Type: Article Affiliation country: Brazil