Oligobodies: bench made synthetic antibodies
Medicina (B.Aires)
;
59(6): 753-8, 1999.
Article
in English
| LILACS
| ID: lil-253534
RESUMO
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA suquence from a database provides enough information to make them.
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Index:
LILACS (Americas)
Main subject:
Oligonucleotides
/
Phosphoprotein Phosphatases
/
Peptide Library
/
Antibody Formation
Limits:
Animals
Language:
English
Journal:
Medicina (B.Aires)
Journal subject:
Medicine
Year:
1999
Type:
Article
Affiliation country:
Brazil
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