Adhesive properties of an outer structure of Clostridium perfrigens type A isolated from piglets with catarrhal enteritis
Rev. microbiol
;
30(3): 242-8, jul.-set. 1999. ilus, tab
Article
in Portuguese, English
| LILACS
| ID: lil-253779
ABSTRACT
One strain (S32) of Clostridium perfringens type A was isolated from a case of catarrhal enteritis of piglets. This strain was able to adhere to HeLa cells showing an adherence index (AI) of 25.15ñ1.26(mean ñ 1 standard error of the mean). Treatment of the bacterial cells with trypsin (025mg/ml) decreased in 70 (per cent)-80 (per cent) the AI and metaperiodate (10mg/ml) abolished completely the adherence, suggesting that the structure responsible for this phenomenon was probably a glycoprotein. Heating of bacterial suspensions (100§C/5 min) before carrying out the adhesion test decreased the AI rendering it equal to the negative controls. Rabbit homologous S32 antiserum inhibited the adherence up to dilutions of 1640, at least. The piglet ileal loop assay carried out with strains S32 and Jab-1 (negative control) demonstrated that the strain S32 was able to adhere to the intestinal epithelial cells when examined after Gram staining. Transmission electron microscopy (TEM) demonstrated that S32 strain displayed a loose fibrillar material not seen with Jab-1. Stabilization of the bacterial cells with homologous antiserum of strain S32, followed by staining with rhutenium red, revealed loose long fibrillar material on the outer surface of the cells, that sometimes could be seen spreading out from the cells and linking bacterial cells. The question whether this structure might be an adhesin for this strain of Cl. perfringes type A, perhaps playing a role in the pathogenesis of the catarrhal enteritis of piglets, is dependent on further studies.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Clostridium Infections
/
Clostridium perfringens
/
Enteritis
Limits:
Animals
Language:
English
/
Portuguese
Journal:
Rev. microbiol
Journal subject:
Microbiology
Year:
1999
Type:
Article
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