Cloning and sequence analysis of a trypanosoma cruzi alpha-butulin cDNA
Biol. Res
;
30(4): 161-6, 1997. ilus, graf
Article
in English
| LILACS
| ID: lil-255657
ABSTRACT
A cDNA clone derived from the Trypanosoma cruzi alpha-tubulin gene was isolated and sequenced (Tc alpha tub; L37345). Tc alpha tub revealed an 87.79 percent and an 85.36 percent identity with the DNA sequence of T. brucei and Leishmania, respectively. This clone was used to study, by Northern blots, alpha-tubulin gene expression in epimastigotes, cell-cultured derived trypomastigotes and extracellular amastigotes. alpha-tubulin MRNA levels were the same in epimastigotes and trypomastigotes, however, there was a drastic decrease in amastigotes. This clone could be useful to elucidate the regulatory mechanisms of alpha-tubulin gene expression during the differentiation of T. cruzi
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Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Tubulin
/
RNA, Messenger
/
Sequence Homology, Nucleic Acid
/
Gene Expression
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
DNA, Complementary
/
Nucleotides
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
1997
Type:
Article
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