Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Rev. microbiol
;
30(2): 120-4, abr.-jun. 1999. tab, graf
Article
in Portuguese, English
| LILACS
| ID: lil-257206
ABSTRACT
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0,8,24,59,62 and 67. The amount of toxin at each injection was 5,6,20,50,100 and 200ug, respectively. The total amount of toxin was 381ug, which corresponded to a reduction of 107ug in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 160 and using ELISA the titer was 1100.000. The lack of cross-reactivity with other staphylococcal enterotoxins indicated high specificity of the antibody to SEA. The proposed immunization scheme was adequate to produce specific SEA antisera, with high titers and the aditional advantage of reducing the amount of purified SEA required for immunization.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Staphylococcus aureus
/
Enterotoxins
/
Antibodies, Bacterial
Type of study:
Health economic evaluation
Limits:
Animals
Language:
English
/
Portuguese
Journal:
Rev. microbiol
Journal subject:
Microbiology
Year:
1999
Type:
Article
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