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Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Sircili, Marcelo Palma; Roxo, Eliana; Leão, Sylvia Cardoso.
  • Sircili, Marcelo Palma; Escola Paulista de Medicina. Universidade Federal São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia.
  • Roxo, Eliana; Instituto Biológico. Seção de Patologia Clínica.
  • Leão, Sylvia Cardoso; Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia.
Rev. microbiol ; 30(2): 144-8, abr.-jun. 1999. ilus, tab
Article in Portuguese, English | LILACS, SES-SP | ID: lil-257211
ABSTRACT
Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
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Full text: Available Index: LILACS (Americas) Main subject: Mycobacterium avium Complex Type of study: Prognostic study Limits: Humans Language: English / Portuguese Journal: Rev. microbiol Year: 1999 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Mycobacterium avium Complex Type of study: Prognostic study Limits: Humans Language: English / Portuguese Journal: Rev. microbiol Year: 1999 Type: Article