Enhancement of Gamma-linolenic acid production by the fungus Mucor SP LB-54 by growth temperature
Rev. microbiol
;
30(2): 170-5, abr.-jun. 1999. tab, graf
Article
in Portuguese, English
| LILACS
| ID: lil-257215
RESUMO
As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiemnt was capable to accumulate a relatively high amount of intracellular lipid, 20,73 per cent of dry cell weight, and GLA content of 15 per cent of total fatty acids after 5 days of incubation at 28 degree C. As the growth temperature was decreased from 28 to 12 degree C the percentage of GLA increased from 15 to 24 per cent of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production of (GLA) (74 mg/l) was obtained from the Mucor sp LB-54 strain after 5 days of incubation at 28 degree C in basal medium following glucose addition (7 per cent w/v) and incubation for an additional 3 days at 12 degree C. The identity of GLA found in the strin of Mucor sp LB-54 was confirmed by the coupled gas chromatography-mass spectrometry.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Temperature
/
Gamma-Linolenic Acid
/
Mucor
Language:
English
/
Portuguese
Journal:
Rev. microbiol
Journal subject:
Microbiology
Year:
1999
Type:
Article
Affiliation country:
Brazil
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