Chlamydia pneumoniae e aterosclerose: identificaçäo do DNA bacteriano na parede arterial / Chlamydia pneumoniae and atherosclerosis: identification of bacterial DNA in the arterial wall
Arq. bras. cardiol
;
74(2): 119-28, Jan. 2000. tab
Article
in Portuguese, English
| LILACS
| ID: lil-262345
ABSTRACT
OBJECTIVE:
The intracellular Gram-negative bacterium Chlamydia pneumoniae has been associated with atherosclerosis. The presence of Chlamydia pneumoniae has been investigated in fragments of the arterial.METHODS:
Arterial fragments obtained from vascular surgical procedures in 58 patients were analyzed. From these patients, 39 were males and the mean age was 65ñ6 years. The polymerase chain reaction was used to identify the bacterial DNA with a pair of primers that codify the major outer membrane protein (MOMP) of Chlamydia pneumoniae. The amplified product was visualized by electrophoresis in the 2 porcento agarose gel stained with ethidium bromide, and it was considered positive when migrating in the band of molecular weight of the positive controls.RESULTS:
Seven (12 per cent) out of the 58 patients showed positive results for Chlamydia pneumoniae.CONCLUSION:
DNA from Chlamydia pneumoniae was identified in the arterial wall of a substantial number of patients with atherosclerosis. This association, which has already been described in other countries, corroborates the evidence favoring a role played by Chlamydia pneumoniae in therogenesis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Bacterial
/
Chlamydia Infections
/
Bacterial Typing Techniques
/
Chlamydophila pneumoniae
/
Atherosclerosis
Type of study:
Diagnostic study
/
Etiology study
/
Prognostic study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Language:
English
/
Portuguese
Journal:
Arq. bras. cardiol
Journal subject:
Cardiology
Year:
2000
Type:
Article
Affiliation country:
Brazil
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