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Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
Espíndola, Noeli M; De Gaspari, Elizabeth N; Nakamura, Paulo M; Vaz, Adelaide J.
  • Espíndola, Noeli M; Universidade de São Paulo. Faculdade de Ciências Farmacêuticas.
  • De Gaspari, Elizabeth N; Instituto Adolfo Lutz.
  • Nakamura, Paulo M; Instituto Adolfo Lutz.
  • Vaz, Adelaide J; Universidade de São Paulo. Faculdade de Ciências Farmacêuticas.
Rev. Inst. Med. Trop. Säo Paulo ; 42(3): 175-7, May-Jun. 2000. ilus
Article in English | LILACS | ID: lil-262699
ABSTRACT
We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.
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Full text: Available Index: LILACS (Americas) Main subject: Taenia / Antibodies, Helminth / Antibodies, Monoclonal / Antigens, Helminth Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2000 Type: Article Affiliation country: Brazil

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Full text: Available Index: LILACS (Americas) Main subject: Taenia / Antibodies, Helminth / Antibodies, Monoclonal / Antigens, Helminth Limits: Animals Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2000 Type: Article Affiliation country: Brazil