Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Braz. j. med. biol. res
;
33(8): 889-95, Aug. 2000. tab, ilus
Article
in English
| LILACS
| ID: lil-265845
RESUMO
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4 percent casein as compared to 20 percent casein in the control diet. When the experimental group had attained a 20 percent loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5 percent) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bone Marrow Cells
/
Fibronectins
/
Laminin
/
Protein-Energy Malnutrition
/
Extracellular Matrix
Type of study:
Observational study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2000
Type:
Article
/
Congress and conference
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Ciências da Saúde/BR
/
Universidade Federal de Santa Catarina/BR
/
Universidade de São Paulo/BR
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