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Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
Aragão, T. C. F. R; Souza, P. A. S; Uchôa, A. F; Costa, I. R; Bloch Júnior, C; Campos, F. A. P.
  • Aragão, T. C. F. R; Universidade Estadual do Ceará. Departamento de Biologia. BR
  • Souza, P. A. S; Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. BR
  • Uchôa, A. F; Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. BR
  • Costa, I. R; Universidade Federal do Ceará. Departamento de Bioquímica e Biologia Molecular. BR
  • Bloch Júnior, C; Universidade de Brasília. Instituto de Química. BR
  • Campos, F. A. P; Universidade Estadual do Ceará. Departamento de Biologia. BR
Braz. j. med. biol. res ; 33(8): 897-903, Aug. 2000. ilus, tab
Article in English | LILACS | ID: lil-265846
RESUMO
We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed
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Full text: Available Index: LILACS (Americas) Main subject: Plant Proteins / Seeds / Albumins / Methionine Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Ceará/BR / Universidade Federal do Ceará/BR / Universidade de Brasília/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plant Proteins / Seeds / Albumins / Methionine Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual do Ceará/BR / Universidade Federal do Ceará/BR / Universidade de Brasília/BR