Chitinolytic activity in viable spores of encephalitozoon species
Mem. Inst. Oswaldo Cruz
;
95(5): 701-5, Sept.-Oct. 2000. graf
Article
in English
| LILACS
| ID: lil-267897
RESUMO
By employing 4-methylumbelliferyl-beta-D-NN',N"-triacetylchitotriose substrate in a semi quantitative assay, chitinolytic activity in viable spores of Encephalitozoon cuniculi and E. intestinalis was detected and dependence on reaction time, spore concentration, concentration of substrate and temperature were demonstrated. It was possible to block the chitinolytic activity by chitin hydrolysate. By incubation at 80§C for 10 min or at 55§C for 20 min the spores were loosing the chitinolytic activity. Incubation of the spores in trypsin reduced the chitinolytic activity. Cellulase activity could not be detected.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Chitinases
/
Encephalitozoon
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2000
Type:
Article
Affiliation country:
Brazil
/
Germany
Institution/Affiliation country:
Bernhard-Nocht-Institute for Tropical Medicine/DE
/
Instituto Oswaldo Cruz/BR
Similar
MEDLINE
...
LILACS
LIS