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Effect of collection, transport, processing and storage of blood specimens on the activity of lysosomal enzymes in plasma and leukocytes
Burin, M; Dutra Filho, C; Brum, J; Mauricio, T; Amorim, M; Giugliani, R.
  • Burin, M; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. BR
  • Dutra Filho, C; Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica. Porto Alegre. BR
  • Brum, J; Hospital Sarah Kubischeck. Brasília. BR
  • Mauricio, T; Universidade Federal da Paraíba. Serviço de Genética Médica. Joao Pessoa. BR
  • Amorim, M; Universidade Federal da Pará. Departamento de Genética. Belém. BR
  • Giugliani, R; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Serviço de Genética Médica. BR
Braz. j. med. biol. res ; 33(9): 1003-13, Sept. 2000.
Article in English | LILACS | ID: lil-267963
RESUMO
This study was designed to evaluate the effect of different conditions of collection, transport and storage on the quality of blood samples from normal individuals in terms of the activity of the enzymes Beta-glucuronidase, total hexosaminidase, hexosaminidase A, arylsulfatase A and Beta-galactosidase. The enzyme activities were not affected by the different materials used for collection (plastic syringes or vacuum glass tubes). In the evaluation of different heparin concentrations (10 percent heparin, 5 percent heparin, and heparinized syringe) in the syringes, it was observed that higher doses resulted in an increase of at least 1-fold in the activities of Beta-galactosidase, total hexosaminidase and hexosaminidase A in leukocytes, and Beta-glucuronidase in plasma. When the effects of time and means of transportation were studied, samples that had been kept at room temperature showed higher deterioration with time (72 and 96 h) before processing, and in this case it was impossible to isolate leukocytes from most samples. Comparison of heparin and acid citrate-dextrose (ACD) as anticoagulants revealed that Beta-glucuronidase and hexosaminidase activities in plasma reached levels near the lower normal limits when ACD was used. In conclusion, we observed that heparin should be used as the preferable anticoagulant when measuring these lysosomal enzyme activities, and we recommend that, when transport time is more than 24 h, samples should be shipped by air in a styrofoam box containing wet ice
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Blood Specimen Collection / Cerebroside-Sulfatase / Glycoside Hydrolases / Leukocytes / Lysosomes Limits: Adolescent / Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Sarah Kubischeck/BR / Universidade Federal da Paraíba/BR / Universidade Federal da Pará/BR / Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Blood Specimen Collection / Cerebroside-Sulfatase / Glycoside Hydrolases / Leukocytes / Lysosomes Limits: Adolescent / Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Sarah Kubischeck/BR / Universidade Federal da Paraíba/BR / Universidade Federal da Pará/BR / Universidade Federal do Rio Grande do Sul/BR