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Combined chromatographic methodology for antidoping control in horse urine
Salvadori, Myriam C; Azevedo, Cristina P; Rieser, Evelyn M; Ribeiro-Neto, Luciane M; Camargo, Marcia M. A; Andraus, Maristela H; Souza, Mirtes E. V; Lopez, Nancy M. R; Palermo Neto, Joäo.
  • Salvadori, Myriam C; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Azevedo, Cristina P; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Rieser, Evelyn M; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Ribeiro-Neto, Luciane M; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Camargo, Marcia M. A; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Andraus, Maristela H; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Souza, Mirtes E. V; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Lopez, Nancy M. R; Jockey Club de Säo Paulo. Laboratório Antidoping. Säo Paulo. BR
  • Palermo Neto, Joäo; Universidade de Säo Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia Experimental e Comparada. Säo Paulo. BR
Rev. bras. toxicol ; 13(1): 11-24, jul. 2000. tab
Article in English | LILACS | ID: lil-271855
RESUMO
A combination of chromatographic techniques is described for the screening of drugs in horse urine for antidoping control purposes. The procedure consists of liquid-liquid base extraction with analysis by HPTLC and GC-NPD and liquid-liquid acidic extraction followed by HPTLC and HPLC. The results of 86 drugs analyzed by these procedures, as well as limits of detection of the drugs in each technique are presented, The use of ELISA tests are recommended for the screening of drugs which would not be detected by the chromatographic proposed procedure. The results show that the proposed combination of techniques enables an efficient antidoping control of racing horses.
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Index: LILACS (Americas) Main subject: Chromatography / Doping in Sports / Horses Limits: Animals Language: English Journal: Rev. bras. toxicol Journal subject: Toxicology Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Jockey Club de Säo Paulo/BR / Universidade de Säo Paulo/BR

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Index: LILACS (Americas) Main subject: Chromatography / Doping in Sports / Horses Limits: Animals Language: English Journal: Rev. bras. toxicol Journal subject: Toxicology Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Jockey Club de Säo Paulo/BR / Universidade de Säo Paulo/BR