Your browser doesn't support javascript.
loading
Polymerase chain reaction for the laboratory diagnosis of aseptic meningitis and encephalitis
Chesky, Marisa; Scalco, Rosana; Read, Steven; Jobim, Luiz Fernando; Failace, Luciane.
  • Chesky, Marisa; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Unidade de Imunologia. Porto Alegre. BR
  • Scalco, Rosana; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Unidade de Imunologia. Porto Alegre. BR
  • Read, Steven; Public Health Laboratory. Oxford. GB
  • Jobim, Luiz Fernando; Universidade Federal do Rio Grande do Sul. Departamento de Medicina Internal. Porto Alegre. BR
  • Failace, Luciane; Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Unidade de Imunologia. Porto Alegre. BR
Arq. neuropsiquiatr ; 58(3B): 836-42, Sept. 2000.
Article in English | LILACS | ID: lil-273108
ABSTRACT
A protocol for testing cerebrospinal fluid specimens using a range of PCR assays for the diagnosis of central nervous system infection was developed and used to test prospectively 383 specimens. PCR assays were used for the detection of adenovirus, Borrelia burgdorferi, enteroviruses, Epstein Barr virus, cytomegalovirus, herpes simplex virus, human herpes virus type 6, JC virus, Leptospira interrogans, Listeria monocytogenes, lymphocytic choriomeningitis virus, measles virus, mumps virus, Mycobacterium sp., Mycoplasma pneumoniae, Toxoplasma gondii and varicella zoster virus. Of the 383 specimens tested in this study, 46 (12.0 percent) were found to be positive. The microorganisms detected were CMV, enterovirus, Epstein Barr virus, herpes simplex virus, human herpes virus type 6, JC virus, L. monocytogenes, Mycobacterium genus, Toxoplasma gondii and varicella zoster virus. The introduction of the PCR protocol described has improved the diagnosis of a range of central nervous system infections in our laboratory. We believe however that further evaluation of these assays in immunocompromised patients is necessary to better determine the predictive value of positive PCR results in these patient groups
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Polymerase Chain Reaction / Encephalitis / Meningitis, Aseptic Type of study: Diagnostic study / Etiology study / Practice guideline / Prognostic study Limits: Adolescent / Adult / Child / Child, preschool / Humans Language: English Journal: Arq. neuropsiquiatr Journal subject: Neurology / Psychiatry Year: 2000 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Public Health Laboratory/GB / Universidade Federal do Rio Grande do Sul/BR

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: LILACS (Americas) Main subject: Polymerase Chain Reaction / Encephalitis / Meningitis, Aseptic Type of study: Diagnostic study / Etiology study / Practice guideline / Prognostic study Limits: Adolescent / Adult / Child / Child, preschool / Humans Language: English Journal: Arq. neuropsiquiatr Journal subject: Neurology / Psychiatry Year: 2000 Type: Article Affiliation country: Brazil / United kingdom Institution/Affiliation country: Public Health Laboratory/GB / Universidade Federal do Rio Grande do Sul/BR