A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors
Mem. Inst. Oswaldo Cruz
;
95(6): 863-6, Nov.-Dec. 2000. ilus
Article
in English
| LILACS
| ID: lil-273442
ABSTRACT
Due to the overlapping distribution of Trypanosoma rangeli and T. cruzi in Central and South America, sharing several reservoirs and triatomine vectors, we herein describe a simple method to collect triatomine feces and hemolymph in filter paper for further detection and specific characterization of these two trypanosomes. Experimentally infected triatomines feces and hemolymph were collected in filter paper and specific detection of T. rangeli or T. cruzi DNA by polymerase chain reaction was achieved. This simple DNA collection method allows sample collection in the field and further specific trypanosome detection and characterization in the laboratory
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma
/
DNA
/
Polymerase Chain Reaction
/
Triatominae
/
Insect Vectors
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2000
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Centro de Pesquisas René Rachou-Fiocruz/BR
/
Universidade Federal de Santa Catarina/BR
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