A theoretical model of the tridimensional structure of Bacillus thuringiensis subsp. medellin Cry 11Bb toxin deduced by homology modelling
Mem. Inst. Oswaldo Cruz
;
96(3): 357-364, Apr. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-282845
RESUMO
Cry11Bb is an insecticidal crystal protein produced by Bacillus thuringiensis subsp. medellin during its stationary phase; this Â-endotoxin is active against dipteran insects and has great potential for mosquito borne disease control. Here, we report the first theoretical model of the tridimensional structure of a Cry11 toxin. The tridimensional structure of the Cry11Bb toxin was obtained by homology modelling on the structures of the Cry1Aa and Cry3Aa toxins. In this work we give a brief description of our model and hypothesize the residues of the Cry11Bb toxin that could be important in receptor recognition and pore formation. This model will serve as a starting point for the design of mutagenesis experiments aimed to the improvement of toxicity, and to provide a new tool for the elucidation of the mechanism of action of these mosquitocidal proteins
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacillus thuringiensis
/
Bacterial Toxins
/
Insecta
/
Models, Theoretical
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Corporación para Investigaciones Biológica/CO
/
Universidad Pontificia Bolivariana/CO
Similar
MEDLINE
...
LILACS
LIS