Comparison of the fluorescent polarization (TDx) and the enzymatic competitive (EMIT 2000) immune assays for the measurement of cyclosporin A blood concentration
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
;
55(6): 207-212, Nov.-Dec. 2000. tab, graf
Article
in English
| LILACS
| ID: lil-283234
RESUMO
Evaluation of Cyclosporin A (CyA) blood concentration is imperative in solid organ transplantation in order to achieve maximal immunosuppression with the least side effects. We compared the results of whole blood concentrations of CyA in 50 blood samples simultaneously evaluated by the fluorescent polarization immune assay (TDx) and the enzymatic competitive immune assay (EMIT 2000). There was a strong correlation between both kits for any range of CyA blood concentration (R=0.99, p<0.001). The within-run and between-days coefficient of variation were less than 4 percent for both assays. The cost for each CyA measurement was 50 percent lower for the EMIT assay when compared to the TDx assay. We concluded that the EMIT is as accurate as the TDx in measuring CyA blood concentration and has the advantage of a lower cost, as well as the possibility of widespread access to the EMIT methodology in contrast to the TDx equipment, allowing the laboratory to perform several routines within a working day
Full text:
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Index:
LILACS (Americas)
Main subject:
Fluorescence Polarization Immunoassay
/
Immunoenzyme Techniques
/
Kidney Transplantation
/
Cyclosporine
Type of study:
Health economic evaluation
Limits:
Humans
Language:
English
Journal:
Rev. Hosp. Clin. Fac. Med. Univ. Säo Paulo
Journal subject:
Medicine
Year:
2000
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Hospital Sírio Libanes/BR
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