Detection of Toxoplasma gondii antigen in cerebrospinal fluid samples using a dot-enzyme-linked immunosorbent assay
Braz. j. infect. dis
;
1(4): 177-81, Aug. 1997.
Article
in English
| LILACS
| ID: lil-284605
ABSTRACT
Toxoplasma encephalitis (TE) is the most common manifestations of a recurrent Toxoplasma gondii infection in immunocompromised individuals, especially in AIDS patients. The infection is associated with considerable morbidity and mortality, even with improved diagnostic procedures and adequate treatment. The diagnosis of TE is difficult to establish by the demonstration of the parasite in the central nervous system or in cerebrospinal fluid (CSF). In order to diagnose TE we have developed a TGA-Dot-ELISA for antigen detection using rabbit anti-toxoplasma antiserum. We have applied this test to specimens of cerobrospinal fluid from patients with toxoplasma encephalitis (180 patients), neurocysticercosis (15), or bacterial meningitis (15). The relative sensitivity and specificity obtained for the TGA-Dot-ELISA were 0.930 and 0.730, respectively. TGA-Dot-ELISA is a promising method for the rapid diagnosis and effective prophylaxis of toxoplasma encephalitis.
Search on Google
Index:
LILACS (Americas)
Main subject:
Toxoplasma
/
Acquired Immunodeficiency Syndrome
/
Toxoplasmosis, Cerebral
/
AIDS-Related Opportunistic Infections
/
Encephalitis
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
1997
Type:
Article
Similar
MEDLINE
...
LILACS
LIS