Ki-67 expression in anal intraepithelial neoplasia in AIDS
São Paulo med. j
;
119(3): 119-121, May 2001. ilus
Article
in English
| LILACS
| ID: lil-285538
ABSTRACT
CONTEXT AIDS is one of the most important risk factors for progression and recurrence of anogenital condyloma. In a previous work, we observed that patients with warts and high-grade AIN (HAIN) had recurrences more frequently than did patients with warts without AIN. The mechanisms of this increased incidence of high-grade lesions in AIDS are not known. OBJECTIVE:
We studied the expression of the proliferative marker Ki-67 by immunohistochemical methods, in specimens of anal condyloma from HIV+ patients to clarify whether its expression can be associated to the grade of AIN.DESIGN:
A retrospective study of hiltological specimens.SETTING:
University referral unit. SAMPLE 34 patients were divided into two groups (1) condylomas with low grade AIN (LAIN), with 25 patients; and (2) condylomas with HAIN, with 9 patients. In this latter group we examined two areas 2A (HAIN area) and 2B (LAIN area). MAIN MEASUREMENTS The immunohistochemical reaction for Ki-67 was done on histological sections. Slices were lightly stained with hematoxylin, to help us in Ki-67 positive cell counting. The percentage of Ki-67 marked nuclei was calculated. We applied one-way variance analysis for statistics.RESULTS:
The mean number of Ki-67 positive cells in group 1 was 19.68 + or - 10.99; in group 2 (area A) it was 46.73 + or - 10.409; and in area B it was 36.43 + or - 14.731. There were statistical differences between groups 1 and 2A and between groups 1 and 2B. Ki-67 positive cells predominated in the lower layer in LAIN. Positive Ki-67 cells were found in all layers in group 2A, and in group 2B they predominated in the two lower or in all layers of the epithelium.CONCLUSIONS:
Our results suggest that LAIN areas (using routine staining techniques) in HAIN can have a biological behavior more similar to HAIN
Full text:
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Index:
LILACS (Americas)
Main subject:
Anus Neoplasms
/
Precancerous Conditions
/
Tumor Virus Infections
/
Condylomata Acuminata
/
HIV Infections
/
Ki-67 Antigen
Type of study:
Observational study
/
Risk factors
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
São Paulo med. j
Journal subject:
Cirurgia Geral
/
Cincia
/
Ginecologia
/
Medicine
/
Medicina Interna
/
Obstetr¡cia
/
Pediatria
/
Sa£de Mental
/
Sa£de P£blica
Year:
2001
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Adolfo Lutz Institute/BR
/
Emílio Ribas Infectology Institute/BR
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