A comparative study of preservation and storage of Haemophilus influenzae
Mem. Inst. Oswaldo Cruz
;
96(4): 583-586, May 2001. tab, graf
Article
in English
| LILACS
| ID: lil-285550
RESUMO
The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cryopreservation
/
Haemophilus influenzae
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional de Tucumán/AR
Similar
MEDLINE
...
LILACS
LIS