A fucan from the brown seaweed Spatoglossum schröederi inhibits Chinese hamster ovary cell adhesion to several extracellular matrix proteins
Braz. j. med. biol. res
;
34(5): 621-626, May 2001. ilus
Article
in English
| LILACS
| ID: lil-285875
RESUMO
Fucans, a family of sulfated polysaccharides present in brown seaweed, have several biological activities. Their use as drugs would offer the advantage of no potential risk of contamination with viruses or particles such as prions. A fucan prepared from Spatoglossum schröederi was tested as a possible inhibitor of cell-matrix interactions using wild-type Chinese hamster ovary cells (CHO-K1) and the mutant type deficient in xylosyltransferase (CHO-745). The effect of this polymer on adhesion properties with specific extracellular matrix components was studied using several matrix proteins as substrates for cell attachment. Treatment with the polymer inhibited the adhesion of fibronectin to both CHO-K1 (2 x 10(5))()and CHO-745 (2 x 10(5) and 5 x 10(5)) cells. No effect was detected with laminin, using the two cell types. On the other hand, adhesion to vitronectin was inhibited in CHO-K1 cells and adhesion to type I collagen was inhibited in CHO-745 cells. In spite of this inhibition, the fucan did not affect either cell proliferation or cell cycle. These results demonstrate that this polymer is a new anti-adhesive compound with potential pharmacological applications
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polysaccharides
/
Seaweed
/
Cell Adhesion
/
Extracellular Matrix Proteins
/
Anticoagulants
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2001
Type:
Article
/
Congress and conference
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Säo Paulo/BR
/
Universidade Federal do Paraná/BR
/
Universidade Federal do Rio Grande do Norte/BR
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