Erythrophagocytosis assay in patients with autoimmune hemolytic anemia
Medicina (B.Aires)
;
61(1): 49-52, 2001. tab, graf
Article
in English
| LILACS
| ID: lil-286378
RESUMO
The aim of this paper is to evaluate the erythrophagocytosis assay (EA) in patients with autoimmune hemolytic anemia (AIHA). Direct antiglobulin test (DAT), indirect antiglobulin test (ITA) and EA were performed in blood samples from 46 patients with presumed AIHA. The EA was carried out incubating patients'erythocytes and peripheral blood monocyte. A total of 200 monocytes were analysed to determine the percentage of active phagocytic cells (porcentage APC). In 9 of these patients the applied treatment was evaluated by DAT,IAT and EA. In 14 transfusion requirements, the compatibility tets and EA were performed. For EA, patients'monocyte were incubated with erytrocytes from previously selected units sensitized with patients'sera. The porcentage of APC was 32.1 +/- 1.7 in 35 patients with positive DAT and 17.8 +/- 1.3 in 11 patients with negative DAT. This last value was significantly higher than that with negative controls (3.7 +/- 0.3) (p= 0.01). As regards the applied treatment, patients with a successful response (n=6) showed a significant decrease in the initial porcentage APC (31.8 +/- 1.6 to 15.3 +/- 2.4; p= 0.05) while DAT and IAT remained positive. In those patients who required blood transfusion the compatibility test were positive with all the units to be transfused, whereas the porcentage APC varied for each one. Blood units were selected according to the lower porcentage APC.
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Index:
LILACS (Americas)
Main subject:
Phagocytosis
/
Erythrocytes
/
Anemia, Hemolytic, Autoimmune
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Medicina (B.Aires)
Journal subject:
Medicine
Year:
2001
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad Nacional de Rosario/AR
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