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Molecular characterization of Listeria monocytogenes isolated from foods
Pimenta, Fabiana Cristina; Furlanetto, Sirdéia Maura Perrone; Mayer, Leonard W; Timenetsky, Jorge; Santos, Manoel Armando Azevedo dos.
  • Pimenta, Fabiana Cristina; Universidade de Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia.
  • Furlanetto, Sirdéia Maura Perrone; Universidade de Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia.
  • Mayer, Leonard W; Center for Disease Control. Division of Vector-Borne Infection Diseases.
  • Timenetsky, Jorge; Universidade de Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia.
  • Santos, Manoel Armando Azevedo dos; Universidade de Säo Paulo. Instituto de Ciências Biomédicas. Departamento de Microbiologia.
Rev. microbiol ; 30(4): 356-61, out.-dez. 1999. tab
Article in English | LILACS | ID: lil-286791
ABSTRACT
A total of 30 strains of "Listeria monocytogenes" isolated from different foods (16 of different kinds of sausage, 14 cheese) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phospholipase C-PI-PLC enzyme. The "L. monocytogenes" strains were differentiated into six ribotype classes. A total of 13 (43.3(per cent)) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard "L. monocytogenes" prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7(per cent)) strains when incubated at 37ºC, but not at 4ºC. The direct colony hibridizatioon method for hemolysin gene detection showed a positive reaction with other "Listeria" spp. The PI-PLC was produced by (90(per cent)) of the strains analysed. There was no correlation between the six identified ribotypes and the virulence facors (hemolysin and PI-PLC) studied
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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Infections / Food Analysis / Listeria monocytogenes / Meat Products Type of study: Prognostic study Language: English Journal: Rev. microbiol Journal subject: Microbiology Year: 1999 Type: Article

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Infections / Food Analysis / Listeria monocytogenes / Meat Products Type of study: Prognostic study Language: English Journal: Rev. microbiol Journal subject: Microbiology Year: 1999 Type: Article