Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer
Mem. Inst. Oswaldo Cruz
;
96(5): 661-665, July 2001. ilus
Article
in English
| LILACS
| ID: lil-289352
RESUMO
In Cuba, several Biomphalaria species have been reported such as B. orbignyi, B. schrammi, B. helophila, B. havanensis and B. peregrina; only the latter three are considered as potential hosts of Schistosoma mansoni. The specific identification of Biomphalaria species is based on anatomical and morphological characters of genital organs and shells. The correct identification of these snails is complicated by the high variation in these characters, similarity among species and in some cases by the small size of the snails. In this paper, we reported the classical morphological identification, the use of PCR and RFLP analysis of the internal transcribed spacer region of the ribosomal RNA genes for molecular identification of seven snail populations from different localities in Cuba. Using morphological and molecular analysis, we showed that among the studied Cuban Biomphalaria populations only B. havanensis and B. obstructa species were found
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomphalaria
/
Polymorphism, Restriction Fragment Length
/
RNA, Ribosomal
/
Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Caribbean
/
Cuba
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Instituto Ludwig de Pesquisas sobre o Câncer/BR
/
UFMG/BR
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