Comparison between precipitin and ELISA tests in the bloodmeal detection of Aedes aegypti (Linnaeus) and Aedes fluviatilis (Lutz) mosquitoes experimentally fed on feline, canine and human hosts
Mem. Inst. Oswaldo Cruz
;
96(5): 693-695, July 2001. tab
Article
in English
| LILACS
| ID: lil-289359
RESUMO
The identification of arthropod bloodmeals is important in many epidemiological studies, as, the understanding of the life cycle of vectors and the patogens they transmit, as well as helping to define arthropods' control strategies. The precipitin test has been used for decades, but ELISA is slowly becoming more popular. To compare the two tests for sensitivity, specificity and accuracy to detect small insect bloodmeals, Aedes aegypti or Ae. fluviatilis mosquitoes were fed either on feline, canine or human hosts. Mosquitoes were frozen at 6, 12, 24, 48 or 72 h after feeding. Precipitin test showed better specificity and accuracy and ELISA test showed higher sensitivity. Better results with both tests were achieved when mosquitoes were frozen within 48 h from feeding
Full text:
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Index:
LILACS (Americas)
Main subject:
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Precipitin Tests
/
Aedes
/
Eating
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundaçäo do Instituto Oswaldo Cruz/BR
/
Universidade Federal Fluminense/BR
/
Universidade Federal Rural do Rio de Janeiro/BR
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