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A alpha-glycerophosphate dehydrogenase is present in Trypanosoma cruzi glycosomes
Concepcion, J. L; Acosta, H; Quiñones, W; Dubourdieu, M.
  • Concepcion, J. L; Universidad de Los Andes. Facultad de Ciencias. Centro de Ingenería Genética. Unidad de Bioquímica de Parásitos. Mérida. VE
  • Acosta, H; Universidad de Los Andes. Facultad de Ciencias. Centro de Ingenería Genética. Unidad de Bioquímica de Parásitos. Mérida. VE
  • Quiñones, W; Universidad de Los Andes. Facultad de Ciencias. Centro de Ingenería Genética. Unidad de Bioquímica de Parásitos. Mérida. VE
  • Dubourdieu, M; Universidad de Los Andes. Facultad de Ciencias. Centro de Ingenería Genética. Unidad de Bioquímica de Parásitos. Mérida. VE
Mem. Inst. Oswaldo Cruz ; 96(5): 697-701, July 2001. graf, tab
Article in English | LILACS | ID: lil-289360
ABSTRACT
alpha-glycerophosphate dehydrogenase (alpha-GPDH-EC.1.1.1.8) has been considered absent in Trypanosoma cruzi in contradiction with all other studied trypanosomatids. After observing that the sole malate dehydrogenase can not maintain the intraglycosomal redox balance, GPDH activity was looked for and found, although in very variable levels, in epimastigotes extracts. GPDH was shown to be exclusively located in the glycosome of T. cruzi by digitonin treatment and isopycnic centrifugation. Antibody against T. brucei GPDH showed that this enzyme seemed to be present in an essentially inactive form at the beginning of the epimastigotes growth. GPDH is apparently linked to a salicylhydroxmic-sensitive glycerophosphate reoxidizing system and plays an essential role in the glycosome redox balance
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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Glycerolphosphate Dehydrogenase / Microbodies Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2001 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad de Los Andes/VE

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Full text: Available Index: LILACS (Americas) Main subject: Trypanosoma cruzi / Glycerolphosphate Dehydrogenase / Microbodies Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2001 Type: Article / Project document Affiliation country: Venezuela Institution/Affiliation country: Universidad de Los Andes/VE