Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase
Braz. j. med. biol. res
;
34(8): 971-975, Aug. 2001. ilus
Article
in English
| LILACS
| ID: lil-290144
RESUMO
We have examined the role of cell surface glycosaminoglycans in cell division adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental evidence suggest that heparan sulfate proteoglycans play a role in cell adhesion as positive modulators of cell proliferation and as key participants in the process of cell division. Proliferation and cell cycle assays clearly demonstrate that a decrease in the amount of glycosaminoglycans does not inhibit the proliferation of mutant CHO-745 cells when compared to the wild type CHO-K1, in agreement with the findings that both CHO-K1 and CHO-745 cells take 8 h to enter the S phase
Full text:
Available
Index:
LILACS (Americas)
Main subject:
CHO Cells
/
Heparan Sulfate Proteoglycans
/
Extracellular Matrix
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2001
Type:
Article
/
Congress and conference
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Säo Paulo/BR
/
Universidade Federal do Paraná/BR
/
Universidade Federal do Rio Grande do Norte/BR
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