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Partial characterization of nif genes from the bacterium Azospirillum amazonense
Potrich, D. P; Passaglia, L. M. P; Schrank, I. S.
  • Potrich, D. P; Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre. BR
  • Passaglia, L. M. P; Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre. BR
  • Schrank, I. S; Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Porto Alegre. BR
Braz. j. med. biol. res ; 34(9): 1105-1113, Sept. 2001. ilus, tab
Article in English | LILACS | ID: lil-290403
Responsible library: BR1.1
ABSTRACT
Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the nifH and nifD genes) differed from related regions of A. brasilense even in nitrogenase structural genes which are highly conserved among diazotrophic bacteria. The feasibility of the 16S ribosomal RNA gene-based PCR system for specific detection of A. amazonense was shown. Our results indicate that the PCR primers for 16S rDNA defined in this article are highly specific to A. amazonense and can distinguish this species from A. brasilense
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Azospirillum / Nitrogen Fixation Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Azospirillum / Nitrogen Fixation Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal do Rio Grande do Sul/BR