Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells
Braz. j. med. biol. res
;
34(9): 1175-1183, Sept. 2001. ilus, graf
Article
in English
| LILACS
| ID: lil-290404
ABSTRACT
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Angiotensin II
/
Receptors, Angiotensin
/
CHO Cells
/
Endocytosis
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2001
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Säo Paulo/BR
/
Universidade de Säo Paulo/BR
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