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Análisis preliminar de la respuesta IgM específica en la fascioliasis hepática humana mediante inmunoelectrotransferencia / Preliminary report of the specific IgM response in human hepatic fascioliasis by means of immunoelectrotransfer blot
Mercado Pedraza, Rubén; Colombo, Alicia; Torres, Susana.
  • Mercado Pedraza, Rubén; Universidad de Chile. Facultad de Medicina. ICBM. Programa de Parasitología.
  • Colombo, Alicia; Universidad de Chile. Facultad de Medicina. ICBM. Programa de Parasitología.
  • Torres, Susana; Universidad de Chile. Facultad de Medicina. ICBM. Programa de Parasitología.
Rev. chil. tecnol. méd ; 19: 817-20, 1999.
Article in Spanish | LILACS | ID: lil-295209
RESUMO
Enzyme immunoelectrotransfer blot (EITB) has received increasing attention as a confirmative technique for the serodiagnosis of different infectous diseases. Also, EITB has bee used for identifying immunodominant polipeptides as potential antigenic components of parasite's extracts. In this study we used EITB to detect IgM specific antibodies in sera of patients with parasitological confirmed fascioliasis. Our prelininary results show that IgM reactivity was observed in 9 of the 17 sera of infected patients. The sensitivity of EITB was 52.9 percent, with at least 10 IgM immunoreactive bands. Futher studies are needed to define the specificity of the bands observed and to detect IgM immunoreactive polipeptides of Fasciola hepatica in patients with pre-patent fascioliasis. In the parasitological confirmed human infections by F. hepatica the sensitivity of IgM EITB was low and similar to those observed by using IgM ELISA
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Index: LILACS (Americas) Main subject: Immunoblotting / Fascioliasis Type of study: Diagnostic study / Prognostic study Limits: Adolescent / Adult / Female / Humans / Male Language: Spanish Journal: Rev. chil. tecnol. méd Journal subject: Tecnologia Biom‚dica Year: 1999 Type: Article

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Index: LILACS (Americas) Main subject: Immunoblotting / Fascioliasis Type of study: Diagnostic study / Prognostic study Limits: Adolescent / Adult / Female / Humans / Male Language: Spanish Journal: Rev. chil. tecnol. méd Journal subject: Tecnologia Biom‚dica Year: 1999 Type: Article