Concentraciones de leptina y prolactina bioactiva e inmunorreactiva durante el ciclo menstrual normal / Concentrations of lectin and bioactive and immunoreactive prolactine during the normal menstrual cycle

RESUMEN

Se determinaron las concentraciones de leptina durante el ciclo menstrual normal de un grupo (n=19) de mujeres fértiles y las concentraciones diarias de estradiol, HL y HFS. Se precisó la actividad inmunológica de la prolactina por radioinmunoensayo (RIA) y la actividad biológica (BIO), por un bioensayo de proliferación de las células Nb2 de linfoma de rata. Se establecieron las relaciones RIA/BIO para la prolactina. Se encontró un aumento de las concentraciones de prolactina (RIA) coincidente con el pico ovulatorio de HL. Se concluyó que las concentraciones de prolactina bioactiva (BIO) fueron significativamente superiores (p < 0,05) durante las fases periovulatoria y lútea. La correlación RIA/BIO para la prolactina osciló entre 0,8 y 2,8. Las concentraciones de leptina mostraron una gran variabilidad entre los ciclos, pero aumentaron durante la fase lútea temprana de ciclo menstrual, aunque no fueron estadísticamente significativas(AU)

ABSTRACT

The concentrations of leptins during the normal menstrual cycle of a group (n=19) of fertile women and the daily concentrations of estradiol, HL and HFS were determined. The immunological activity of prolactin was determined by radioimmunoassay (RIA), whereas the biological activity (BIO) was determined by a proliferation bioassay of Nb2 cells from rat lymphoma. The RIA/BIO relations for prolactin were established. It was found an increase of the concentrations of prolactin (RIA) coinciding with the HL ovulatory peak. It was concluded that the concentrations of bioactive prolactin (BIO) were significantly higher (p<0.05) during the periovulatory and luteal phases. The RIA/BIO correlation for prolactine oscillated between 0.8 and 2.8. The concentrations of leptine showed a great variability between cycles, but they increased during the early luteal phase of the menstrual cycle, although they were not statistically significant(AU)