Protein profile analysis by SDS-Page of "Mycoplasma gallisepticum" strains S6(208) and F-K810 grown in Hayflick's and Frey's media

Ferraz, Priscila N; Menezes, Cláudio C. P; Danelli, Maria das Graças M; Lizeu, Jackeline de O. P; Nascimento, Elmiro R. do; Lucchesi, Daniela M

Ferraz, Priscila N; Menezes, Cláudio C. P; Danelli, Maria das Graças M; Lizeu, Jackeline de O. P; Nascimento, Elmiro R. do; Lucchesi, Daniela M.

Ferraz, Priscila N; Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Menezes, Cláudio C. P; Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Danelli, Maria das Graças M; Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Lizeu, Jackeline de O. P; Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Nascimento, Elmiro R. do; Universidade Federal Fluminense. Faculdade de Veterinária.
Lucchesi, Daniela M; FIOCRUZ. Instituto de Tecnologia em Imunobiológicos.

Braz. j. microbiol ; 31(2): 113-5, Apr.-Jun. 2000. ilus, graf

Article in English | LILACS | ID: lil-297648

RESUMO

RESUMO

The "Mycoplasma gallisepticum" strains [wild-type S6(208) and vaccine-type F-K810] grown in Frey's and hayflick's media were analysed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.

Subject(s)

Mycoplasma/enzymology; Proteins/analysis; Densitometry

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Full text: Available Index: LILACS (Americas) Main subject: Proteins / Mycoplasma Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2000 Type: Article Affiliation country: Brazil

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