Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil
Braz. j. microbiol
;
31(2): 140-5, Apr.-Jun. 2000. ilus, tab
Article
in English
| LILACS
| ID: lil-297653
RESUMO
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immonuassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus one samples was group A / soubgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any primer used. Two electropherotypes were detected and both were present in the same animal. This study demonstrates that two different electropherotypes and/or serotypes of bovine rotavirus can circulate in the same outbreak.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Rotavirus Infections
/
Rotavirus
/
Diarrhea Viruses, Bovine Viral
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2000
Type:
Article
Affiliation country:
Brazil
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