Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques
Braz. j. microbiol
;
32(1): 27-31, Jan.-Mar. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-297663
RESUMO
Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7 per cente of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds.(au)
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Staphylococcus aureus
/
Polymerase Chain Reaction
/
Mastitis, Bovine
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2001
Type:
Article
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