Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism
Mem. Inst. Oswaldo Cruz
;
96(6): 809-813, Aug. 2001. ilus, graf
Article
in English
| LILACS
| ID: lil-298602
ABSTRACT
Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70 percent of the samples. PCR confirmed the identification of 23 samples (100 percent) that grew in culture, 9 samples (60 percent) that failed to grow in culture, plus 6 (37.5 percent) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Lymph Nodes
/
Mycobacterium bovis
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Argentina
/
Brazil
Institution/Affiliation country:
Centro de Investigación en Ciencias Veterinarias/AR
/
Escola Paulista de Medicina/BR
/
ICB/BR
/
Instituto Biológico de Säo Paulo/BR
/
Laboratório Central do Estado do Espírito Santo/BR
/
Universidade Federal de Minas Gerais/BR
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