A simple modification of the Baermann method for diagnosis of strongyloidiasis
Mem. Inst. Oswaldo Cruz
;
96(6): 805-807, Aug. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-298616
RESUMO
The diagnosis of Strongyloides stercoralis infections is routinely made by microscopic observation of larvae in stool samples, a low sensitivity method, or by other, most effective methods, such as the Baermann or agar culture plate methods. We propose in this paper a practical modification of Baermann method. One hundred and six stool samples from alcoholic patients were analyzed using the direct smear test, agar culture plate method, the standard Baermann method, and its proposed modification. For this modification the funnel used in the original version of the method is substituted by a test tube with a rubber stopper, perforated to allow insertion of a pipette tip. The tube with a fecal suspension is inverted over another tube containing 6 ml of saline solution and incubated at 37ÝC for at least 2 h. The saline solution from the second tube is centrifuged and the pellet is observed microscopically. Larva of S. stercoralis were detected in six samples (5.7 percent) by the two versions of the Baermann method. Five samples were positive using the agar culture plate method, and only in two samples the larva were observed using direct microscopic observation of fecal smears. Cysts of Endolimax nana and Entamoeba histolytica/dyspar were also detected in the modification of Baermann method. Data obtained by the modified Baermann method suggest that this methodology may helps concentrate larvae of S. stercoralis as efficiently as the original method:
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Strongyloides
/
Strongyloidiasis
/
Feces
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Costa Rica
Institution/Affiliation country:
Universidad de Costa Rica/CR
Similar
MEDLINE
...
LILACS
LIS