Your browser doesn't support javascript.
loading
Lack of mutations of exon 2 of the MEN1 gene in endocrine and nonendocrine sporadic tumors
Costa, S. C; Nascimento, L. S; Ferreira, F. J; Mattos, P. S; Camara-Lopes, L. H; Ward, L. S.
  • Costa, S. C; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica. Campinas. BR
  • Nascimento, L. S; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica. Campinas. BR
  • Ferreira, F. J; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica. Campinas. BR
  • Mattos, P. S; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Anatomia Patológica. Campinas. BR
  • Camara-Lopes, L. H; Hospital Sírio Libanês de Säo Paulo. Departamento de Patologia. Säo Paulo. BR
  • Ward, L. S; Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Clínica Médica. Campinas. BR
Braz. j. med. biol. res ; 34(7): 861-865, July 2001. ilus, tab
Article in English | LILACS | ID: lil-298670
ABSTRACT
In addition to the mutations that underlie most cases of the multiple endocrine neoplasia type 1 (MEN1) syndrome, somatic mutations of the MEN1 gene have also been described in sporadic tumors like gastrinomas, insulinomas and bronchial carcinoid neoplasm. We examined exon 2 of this gene, where most of the mutations have been described, in 148 endocrine and nonendocrine sporadic tumors. DNA was obtained by phenol/chloroform extraction and ethanol precipitation from 92 formalin-fixed, paraffin-embedded samples, and from 40 fresh tumor tissue samples. We used 5 pairs of primers to encompass the complete coding sequence of exon 2 of the MEN1 gene that was screened by the polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) technique in 78 sporadic thyroid cancers 28 follicular adenomas, 35 papillary carcinomas, 14 follicular carcinomas, and 1 anaplastic thyroid carcinoma. We also examined 46 adrenal lesions (3 hyperplasias, 3 adenomas and 35 adrenocortical carcinomas, 2 pheochromocytomas, 2 ganglioneuroblastomas, and 1 lymphoma) and 24 breast cancers (6 noninvasive, 16 infiltrating ductal, and 2 invasive lobular tumors). The PCR product of 5 tumors suspected to present band shifts by SSCP was cloned. Direct sense and antisense sequencing did not identify mutations. These results suggest that the MEN1 gene is not important in breast, thyroid or adrenal sporadic tumorigenesis. Because the frequency of mutations varies significantly among tumor subgroups and allelic deletions are frequently observed at 11q13 in thyroid and adrenal cancers, another tumor suppressor gene residing in this region is likely to be involved in the tumorigenesis of these neoplasms
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / DNA, Neoplasm / Exons / Adrenal Gland Neoplasms / Multiple Endocrine Neoplasia Type 1 / Mutation Limits: Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Sírio Libanês de Säo Paulo/BR / Universidade Estadual de Campinas/BR

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: LILACS (Americas) Main subject: Breast Neoplasms / DNA, Neoplasm / Exons / Adrenal Gland Neoplasms / Multiple Endocrine Neoplasia Type 1 / Mutation Limits: Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2001 Type: Article Affiliation country: Brazil Institution/Affiliation country: Hospital Sírio Libanês de Säo Paulo/BR / Universidade Estadual de Campinas/BR