Infiltrating ductal mammary carcinoma: lectin Histochemistry study

RESUMO

Parkia pendula seed lectin (PpeL) was evaluated as histochemical marker for human infiltrating duct carcinoma. Concanavalin A (Con A) was used as pattern of tissue lectin binding. Tissues were treated with 0,1 por cento trypsin solution and 0,3 por cento methanol-H2. Optic microscopy waas used to evaluate the lectin-binding pattern to tissue. Methyl-a-D-mannoside, at 300nM, was used to inhibit the lectin binding to mammary tissues. The inhibition assay reinforced that Ppel conjugated to peroxidase bound to glucose and/or mannose residues in the membrane surface glycoconjugates. Distinct binding patterns of Ppel, associated to different mammary epithelium dedifferentiation, were comparable to those obtained with Con A. These findings indicate a potencial use of this lectin as histochemical marker in investigations of breast neoplasic alterations