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An indirect immunofluorescence assay to detect antibodies against St. Louis Encephalitis virus
Spinsanti, Lorena; Re, Viviana; Aguilar, Javier; Contigiani, Marta.
  • Spinsanti, Lorena; National University of Cordoba. Faculty of Medical Sciences. Institute of Virology Dr 'J.M. Vanella'. Cordoba. AR
  • Re, Viviana; National University of Cordoba. Faculty of Medical Sciences. Institute of Virology Dr 'J.M. Vanella'. Cordoba. AR
  • Aguilar, Javier; National University of Cordoba. Faculty of Medical Sciences. Institute of Virology Dr 'J.M. Vanella'. Cordoba. AR
  • Contigiani, Marta; National University of Cordoba. Faculty of Medical Sciences. Institute of Virology Dr 'J.M. Vanella'. Cordoba. AR
Rev. Inst. Med. Trop. Säo Paulo ; 43(6): 339-340, Nov.-Dec. 2001. tab
Article in English | LILACS | ID: lil-303045
Responsible library: BR1.1
RESUMO
An in house indirect immmunofluorescence assay ( IFA ) in relation to neutralization (NT) reference test, was assessed as a fast and cheap method to carry out serological surveys for St. Louis Encephalitis virus (SLE). Sera obtained from 213 blood donors were analyzed by both tests. The prevalence of seropositivity obtained with IFA was lower than (30.98 percent) that observed on NT (41.78 percent). The relative specificity rate of IFA was 96.77 percent whereas its relative sensitivity rate was 69.66 percent. Kappa index showed a good correlation between both tests. The results indicate that neutralization assay is still the serological test with the highest sensitivity and specificity relative rates for detecting antibodies against SLE virus. Nevertheless, the IFA could be useful as an alternative test in order to learn the circulation of the Flavivirus genus in a certain area
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Encephalitis Virus, St. Louis / Antibodies, Viral Limits: Humans Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2001 Type: Article Affiliation country: Argentina Institution/Affiliation country: National University of Cordoba/AR

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Full text: Available Index: LILACS (Americas) Main subject: Encephalitis Virus, St. Louis / Antibodies, Viral Limits: Humans Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2001 Type: Article Affiliation country: Argentina Institution/Affiliation country: National University of Cordoba/AR