Bone marrow fibroblasts in patients with advanced lung cancer
Braz. j. med. biol. res
;
34(11): 1457-1463, Nov. 2001. tab
Article
in English
| LILACS
| ID: lil-303323
ABSTRACT
In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of pure fibroblast cultures were measured with an ELISA kit and RIA kit, respectively. Only fibroblasts from 6/13 (46 percent) LCP confluent primary cultures had the capacity to proliferate following four subcultures (NC = 100 percent). Levels of spontaneously released IL-1ß were below 10 pg/ml in the CM of LCP, while NC had a mean value of 1,217 + or - 74 pg/ml. In contrast, levels of PGE2 in these CM of LCP were higher (77.5 + or - 23.6 pg/ml) compared to NC (18.5 + or - 0.9 pg/ml). In conclusion, bone marrow fibroblasts from LCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1ß and PGE2 production
Full text:
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Index:
LILACS (Americas)
Main subject:
Bone Marrow Cells
/
Carcinoma, Non-Small-Cell Lung
/
Fibroblasts
/
Lung Neoplasms
Type of study:
Observational study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2001
Type:
Article
/
Congress and conference
Affiliation country:
Argentina
Institution/Affiliation country:
Hospital Británico/AR
/
I. Iriarte Hospital/AR
/
Instituto de Biología y Medicina Experimental/AR
/
Research Member of the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET)/AR
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