An outbreak of gastroenteritis associated with astrovirus serotype 1 in a day care center, in Rio de Janeiro, Brazil
Mem. Inst. Oswaldo Cruz
;
96(8): 1069-1073, Nov. 2001. ilus, tab
Article
in English
| LILACS
| ID: lil-304642
RESUMO
Between June 4th and June 20th1996 rotavirus, adenovirus, and astrovirus (HAstrV) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the Bertha Lutz day care center, in Rio de Janeiro. All fecal samples were analyzed by polyacrylamide gel electrophoresis (PAGE), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme immunoassays (EIA), and electron microscopy (EM). Nine of them (33 percent) showed positive results for HAstrV by at least one of the employed methodologies. Eight were positive by RT-PCR and EIA, and six by EM. All positive samples were inoculated onto HT-29 (human colon adenocarcinoma) cultured cells for HAstrV isolation and seven were positive after three passages. The sequencing analysis of eight RT-PCR products (449 bp) from gene that codifies VP2 protein, showed a total nucleotide identity among them and 98 percent with HAstrV-1 (strain Oxford type 1). This is the first report of a gastroenteritis outbreak associated with HAstrv-1 in a day care center in Rio de Janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mamastrovirus
/
Child Day Care Centers
/
Disease Outbreaks
/
Astroviridae Infections
/
Gastroenteritis
Type of study:
Diagnostic study
/
Risk factors
Limits:
Child, preschool
/
Humans
/
Infant
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2001
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Oswaldo Cruz-Fiocruz/BR
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