A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium
Braz. j. microbiol
;
31(1): 30-36, jan.-mar. 2000. ilus
Article
in English
| LILACS
| ID: lil-306362
ABSTRACT
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0 (per cent) and 0.5 (per cent), was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on cellular carbohydrate expression is discussed.
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Index:
LILACS (Americas)
Main subject:
Yeasts
/
In Vitro Techniques
/
Candida
/
Carbohydrates
/
Fungi
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2000
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Catolica de Pernambuco/BR
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