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A pratical method for screening for (beta)-galactosidase secreting microbial colonies
Barreto, Eriana S; Silva, Daison O; Passos, Flávia M. Lopes.
  • Barreto, Eriana S; Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa. BR
  • Silva, Daison O; Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa. BR
  • Passos, Flávia M. Lopes; Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa. BR
Braz. j. microbiol ; 31(1): 37-38, jan.-mar. 2000. ilus
Article in English | LILACS | ID: lil-306363
ABSTRACT
Microbial colonies were replicated on YNB© agar plates overlaid with soft agar containing the glucose-oxidase/peroxidase (BIOTROLr) system. The pink color developed around the colonies was the result of the reaction of this enzyme. This method proved to be very convenient for testing hundreds of colonies grown on agar plates for (beta)-galactosidase secretion by microbial cells.
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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Beta-Galactosidase / Enzyme Reactivators / Clinical Enzyme Tests Type of study: Diagnostic study / Screening study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Viçosa/BR

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Full text: Available Index: LILACS (Americas) Main subject: In Vitro Techniques / Beta-Galactosidase / Enzyme Reactivators / Clinical Enzyme Tests Type of study: Diagnostic study / Screening study Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2000 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Viçosa/BR