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Ultrastructural, Antigenic and Physicochemical Characterization of the Mojuí dos Campos (Bunyavirus) Isolated from Bat in the Brazilian Amazon Region
Wanzeller, Ana Lm; Diniz, José Ap; Gomes, Maria Lc; Cruz, Ana Cr; Soares, Manoel Cp; Souza, Wanderley de; Rosa, Amélia Pa Travassos da; Vasconcelos, Pedro Fc.
Affiliation
  • Wanzeller, Ana Lm; Instituto Evandro Chagas. Belém. BR
  • Diniz, José Ap; Instituto Evandro Chagas. Belém. BR
  • Gomes, Maria Lc; Instituto Evandro Chagas. Belém. BR
  • Cruz, Ana Cr; Instituto Evandro Chagas. Belém. BR
  • Soares, Manoel Cp; Instituto Evandro Chagas. Belém. BR
  • Souza, Wanderley de; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular. Rio de Janeiro. BR
  • Rosa, Amélia Pa Travassos da; University of Texas Medical Branch. Department of Pathology. Galveston. US
  • Vasconcelos, Pedro Fc; Instituto Evandro Chagas. Belém. BR
Mem. Inst. Oswaldo Cruz ; 97(3): 307-311, Apr. 2002. ilus
Article in En | LILACS | ID: lil-307965
Responsible library: BR1.1
RESUMO
The Mojuí dos Campos virus (MDCV) was isolated from the blood of an unidentified bat (Chiroptera) captured in Mojuí dos Campos, Santarém, State of Pará, Brazil, in 1975 and considerated to be antigenically different from other 102 arboviruses belonging to several antigenic groups isolated in the Amazon region or another region by complement fixation tests. The objective of this work was to develop a morphologic, an antigenic and physicochemical characterization of this virus. MDCV produces cytopathic effect in Vero cells, 24 h post-infection (p.i), and the degree of cellular destruction increases after a few hours. Negative staining electron microscopy of the supernatant of Vero cell cultures showed the presence of coated viral particles with a diameter of around 98 nm. Ultrathin sections of Vero cells, and brain and liver of newborn mice infected with MDCV showed an assembly of the viral particles into the Golgi vesicles. The synthesis kinetics of the proteins for MDCV were similar to that observed for other bunyaviruses, and viral proteins could be detected as early as 6 h p.i. Our results reinforce the original studies which had classified MDCV in the family Bunyaviridae, genus Bunyavirus as an ungrouped virus, and it may represent the prototype of a new serogroup
Subject(s)
Full text: 1 Index: LILACS Main subject: Bunyaviridae / Chiroptera Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2002 Type: Article
Full text: 1 Index: LILACS Main subject: Bunyaviridae / Chiroptera Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Mem. Inst. Oswaldo Cruz Journal subject: MEDICINA TROPICAL / PARASITOLOGIA Year: 2002 Type: Article