Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat
Rev. Inst. Med. Trop. Säo Paulo
; Rev. Inst. Med. Trop. Säo Paulo;43(5): 247-250, Sept.-Oct. 2001. ilus, tab
Article
in En
| LILACS
| ID: lil-307996
Responsible library:
BR1.1
RESUMO
The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1 percent) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken
Full text:
1
Index:
LILACS
Main subject:
Poultry Products
/
Salmonella
/
DNA, Bacterial
/
Chickens
Type of study:
Diagnostic_studies
/
Guideline
Limits:
Animals
Language:
En
Journal:
Rev. Inst. Med. Trop. Säo Paulo
Journal subject:
MEDICINA TROPICAL
Year:
2001
Type:
Article