Metodología clásica y molecular en la identificación de especies de enterococcus spp / Classic and molecular methodologies for the identification of enterococcus species
Rev. méd. Chile
;
130(1): 45-49, ene. 2002. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-310251
RESUMO
Background:
Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial.Aim:
To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). Material andmethods:
Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR.Results:
Eighty nine percent of isolates were identified as E fócalis, 10.2 percent as E fócium and 3.3 percent as other species.Conclusions:
PCR is a fast and promising technique, useful in the identification of Enterococcus species
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymerase Chain Reaction
/
Enterococcus
Type of study:
Diagnostic study
Limits:
Humans
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2002
Type:
Article
/
Project document
Affiliation country:
Chile
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